WORK PLAN


 

Approaches to be followed:

 

       Quantification and prioritization of stresses using the maturing resources of GIS, climate change forecasting, and crop modelling.

       Monitoring Green House Gas Emission, and Development of agronomic techniques that decrease Green House Gas Emission.

       Field-based screening techniques, supported by laboratory and statistical methods, that efficiently identify genetic variation for mechanisms that stabilize and increase production under stress at no cost to unstressed performance, followed by the optimized use of such secondary traits in selection.

       Efficient combination of conventional breeding methods with gene discovery through expression methodology, genomics and molecular markers to identify, validate and integrate key alleles and genes suited to specific environmental challenges.

       Cross-talk among different abiotic stress response and cis-acting elements in stress inducible promoters.

       Integration of gene expression data and metabolites changes to identify pathways / genes involved in phenotypic appearances.

 

Methodologies to be applied:

 

In addition of standard methodologies some high throughput screening techniques will be applied based on plant phenomics.

 

Methodology in relation to Phenomics:

 

       Infrared thermography imaging and 3D reconstruction of plant phenome

       Hyperspectral imaging  of C, N, and phenolics

       FITR imaging at cellular level

       Chlorophyll fluorescence imaging of photosynthesis

       Hyperspectral  sensing based on NDVI, PRI etc for stress tolerance 

 

I. Thematic Area: Evaluation of key rice germplasm for tolerance to submergence, drought and salinity

 

Objective:

       Identification of key rice germplasm tolerant to anaerobic germination, complete submergence for three weeks or more, water-logging (medium depth, 0-50 cm), drought and salinity using low, medium and high throughput screening techniques.

       Development elite lines tolerant to drought, salinity, salinity with waterlogging/submergence tolerance.

       High through put studies on root system architecture and development under drought.

        Monitoring expression profiles of genes and metabolites in rice cultivars tolerant to more than one abiotic stresses (e.g. salinity & submergence, submergence & drought and drought and salinity) to identify new genes/metabolites.

       Identification of key abiotic stress tolerant traits associated to physiological, molecular and grain yield.

       Employment of molecular markers to study allelic diversity among salt tolerant lines.

       Identification of new QTLs related to drought and salinity tolerance.

       Identification of germplasm lines and key plant physiological traits related to plant performances under multiple abiotic stresses.

       Phenomics in improving biomass and plant productivity of rice adapted to multiple abiotic stresses.

       Effects of elevated CO2 and interacting environmental variables like drought on rice by utilizing FACE technology.

       Registration of germplasm tolerant to different abiotic stresses.

 

Activity Miles stone

 

Sl. No.

Activity Milestone

(Year)

1

2

3

4

5

1

Collection of germplasm grown in coastal saline areas.

 

 

2

Identification of rice germplasm tolerant to anaerobic germination, waterlogging (medium depth, 0-50 cm), complete submergence for three weeks or more, drought and salinity (both vegetative and reproductive stage) tolerance using low, medium and high throughput screening techniques.

3

Development of high yielding drought and salt tolerant varieties for coastal saline and drought prone areas.

4

High through put studies on root system architecture and development under drought.

 

5

Monitoring expression profiles of genes and metabolites in rice cultivars tolerant to more than one abiotic stresses (e.g. salinity & submergence, submergence & drought and drought and salinity) to identify new genes/metabolites.

 

6

Physiological and molecular marker-trait association studies in diverse anaerobic germination, waterlogging and submergence, drought and salinity tolerant rice genotypes.

7

Development of near isogenic lines (NILs) to identify key anaerobic germination, waterlogging, drought and salinity tolerant traits related to physiological, molecular and yield linked qualities.

8

Development of mapping population for identification of QTLs for vegetative /reproductive stage drought and salinity tolerance.

9

Employment of molecular markers to study allelic diversity among salt tolerant lines.

 

10

Identification and development of salt tolerant lines with tolerance to waterlogging/submergence.

 

11

Development of high yielding rice varieties with tolerance to drought through MAB.

12

Phenomics in improving biomass and plant productivity of rice adapted to multiple abiotic stresses.

 

13

Registration of germplasm.

 

 

II. Thematic Area: Improved estimates of GHG emissions in rice based production systems

 

Objectives:

       To estimates the GHG emissions in different rice production systems.

        To estimate GHGs emission in different rice germplasm, and climate stressed condition.

       The diurnal, seasonal and annual fluxes of heat, moisture, CO2 and CH4 would be monitored.

 

Activity Miles stone

  

Sl. No.

Activity Milestone

(Year)

1

2

3

4

5

1

Calibration of eddy covariance system.

 

 

 

 

2

Procurement of GHG sampling chambers, gas cylinders and calibration.

 

 

 

 

3

Data collection and processing of CO2 and CH4 fluxes from Eddy covariance systems

4

Monitoring of GHGs emission in different rice based production systems

5

Monitoring of GHGs emission in different rice germplasm, and  climate stressed condition

 

6

Up-scaling of data in regional scale through modeling and GIS

 

 

 

III. Thematic area: Management practices to reduce emissions from paddy cultivation

 

Objectives:

 

       Evaluation of existing varieties grown in rain fed low land of eastern India for their GHG emission potential and use of  simulation approach for devising an optimum N application schedule for enhanced NUE and reduced N2O emission.

       To evaluate the GHG mitigation potential of different water and nitrogen   management strategy in irrigated rice system and to assess the impact of time and duration of drainage period on N2O and CH4 emission with help of a validated process based biogeochemical model.

       To calibrate and validate a process based biogeochemical model for aerobic rice production system and devising an integrated nutrient management scheme for improved NUE through simulation approach.

 

Activity Miles stone

 

Sl. No.

Activity Milestone

(Year)

1

2

3

4

5

1

Field experiment with selected rice cultivars to measure GHG emission potential and to generate cultivar specific crop coefficients

 

 

 

2

Field experiment with rice under several N application schedule and simulation experiment and selection of best possible N application schedule

 

 

3

Field experiment with rice under different water and nitrogen   management strategy

 

 

4

Simulation experiment with varying time and duration of drainage period.

 

 

5

Field experiment with aerobic rice cultivar to generate cultivar specific crop coefficients and under different N management strategies

 

 

6

Parameterization, calibration and validation of the models

 

7

Testing the recommendations in farmers field

 

 

 

Deliverables:

       Newer germplasms tolerant to anaerobic germination, stagnant water tolerance and three weeks or more tolerant to submergence, drought and salinity would be known.

       Germplasm tolerant to multiple abiotic stresses such as excess water, salinity and drought would be identified.

       Physiological and molecular markers will speed up the conventional breeding as well as biotechnological approaches to develop high yielding rice cultivars adapted to climate change.

       New Genes/QTLs would be discovered.

       High yielding rice varieties tolerant to drought and coastal saline areas are identified.

       Improved quantification of GHGs emission from rice production systems would be known.

        Long-term studies focusing on NEE, RE and GPP as well as productivity of rice will clarify diurnal, seasonal, annual and inter-annual variations in gaseous-C flux dynamics of different rice production systems.

       Characterization of the microclimate in rice canopy of both flooded and aerobic environments in relation to GHG emission would be understood.

       Inter-relationship of the different fluxes of heat, water vapor, CO2 and CH4 would be understood.

       Mitigation of GHG emission from rice fields through different cultivation practices would be possible.

AND Finally promotion of Technology through different stake holders.